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2011年3月9日—5月19日,以厦门海域采集的野生个体和实验室繁育的日本文昌鱼成体为亲本,尝试性地建立了全同胞家系;采用温度和光照诱导的方法,对雌、雄亲本进行人工催产,获得了34对亲本的全同胞家系受精卵。在家系培育过程中发现致使胚胎和仔鱼死亡的几种主要原因,以及日本文昌鱼早期发育的两种模式。通过加强养护管理,提高仔鱼存活率,缩短幼体变态所需时间,最终建立了7个完成变态的全同胞家系。在这些家系中,从受精卵到完成变态后不久的亚成体存活率最高为32.4%,最低为1.67%,而变态最快历时24 d,最慢历时42 d。虽然在全同胞家系建立过程中,幼体死亡率高,家系间的胚胎和幼体生长发育状况差异大,但实验结果表明日本文昌鱼全同胞家系建立完全可行,为其优良品系选育建立了基础。
From March 9 to May 19, 2011, wild-type individuals collected from Xiamen sea area and laboratory-grown Japanese amphioxus adults were tentatively established as whole-sib families. By using temperature and light-induced methods, , The mother of the artificial spawning, obtained 34 parents of all-sib family fertilized eggs. Several major causes of death of embryos and larvae, and two patterns of early development of Japanese amphioxus were identified during pedigree development. By strengthening the conservation and management, raising the survival rate of larvae, shortening the larvae metamorphosis time, and ultimately the establishment of seven metamorphosis of all sib families. Among these pedigrees, the survival rate of adulthood from fertilized egg to shortly after metamorphosis reached the highest was 32.4% and the lowest was 1.67%, while the fastest metamorphosis lasted 24 days and the slowest lasted 42 days. Although the mortality rate of juveniles was high and the growth and development status of embryos and larvae among the families was quite different during the establishment of full sib families, the experimental results showed that the establishment of full sib families in Chinese amphioxus was completely feasible and established the basis for the breeding of its excellent strains.