目的探讨肝细胞刺激因子 (HSS)对正常大鼠肝细胞和人肝癌细胞的作用特点。方法应用自制的HSS分别与正常大鼠肝脏细胞和人传代肝癌细胞共同培养不同时间 ,以促进肝细胞增生的IL 6为对照组 ,采用3 H TdR掺入法 ,观察HSS对大鼠肝细胞和人肝癌细胞分裂增生和细胞形态变化的影响。结果在培养早期HSS对正常大鼠肝细胞和人肝癌细胞均具有刺激增生的作用 ,随后HSS对这些细胞的增生具有明显的抑制作用 ,并伴随着细胞的死亡形态改变。结论HSS对人肝癌细胞和正常大鼠肝细胞的增生不仅有促进作用 ,而且具有较强的抑制作用 ,此对进一步研究HSS的生物学活性和临床应用具有重要意义 Objective To investigate the effect of hepatocyte stimulating factor (HSS) on normal rat hepatocytes and human hepatoma cells. Methods HSCs were co-cultured with normal liver cells and human hepatoma cells in different time to promote the proliferation of hepatocytes IL-6 as the control group, using 3 H TdR incorporation method to observe the effects of HSS on rat hepatocytes and Effects of hepatocellular carcinoma cell division and proliferation and cell morphological changes. Results In the early stage of culture, HSS could stimulate proliferation of normal rat hepatocytes and human hepatocellular carcinoma cells. HSS then significantly inhibited the proliferation of these cells and accompanied the morphological changes of the cells. Conclusion HSS not only promotes the proliferation of human hepatoma cells and normal rat hepatocytes, but also has a strong inhibitory effect, which is of great significance for the further study of the biological activity and clinical application of HSS