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目的建立RP-HPLC法同时测定甘草药材中甘草酸差向异构体的含量。方法采用HypersilC18(250 mm×4.6 mm,5μm)柱,以甲醇-体积分数为1%的醋酸溶液(体积比为56∶44)为流动相,流速为1.0 mL.min-1,检测波长为250 nm,柱温为35℃。结果α-甘草酸和β-甘草酸的线性范围分别为0.010~0.209 g.L-1(r=0.9995)和0.052~1.034 g.L-1(r=0.9996)。2种成分的平均加样回收率(n=9)分别为99.7%和99.4%。结论该方法可用于甘草药材中甘草酸差向异构体的同时含量测定。
OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of glycyrrhizic acid epimers in licorice root. Methods A Hypersil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of a 1% acetic acid solution (volume ratio 56:44) with a flow rate of 1.0 mL · min-1 and a detection wavelength of 250 nm, the column temperature is 35 ℃. Results The linear ranges of α-glycyrrhizin and β-glycyrrhizin were 0.010-0.209 g.L-1 (r = 0.9995) and 0.052-1.034 g.L-1 (r = 0.9996), respectively. The average recoveries (n = 9) for the two components were 99.7% and 99.4%, respectively. Conclusion The method can be used for the simultaneous determination of glycyrrhizic acid epimer in licorice root medicine.