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【目的】探讨过敏性紫癜(henoch-schonlein purpura,HSP)患儿单个核细胞(mononuclear cell,MNCs)凋亡的变化,并检测周围血中细胞因子白细胞介素-8、白细胞介素-6、肿瘤坏死因子-α和胰岛素样生长因子Ⅰ的变化,探讨其对MNCs凋亡的影响。【方法】采用流式细胞术检测36例HSP患儿和26名健康对照者MNCs的凋亡情况,酶联免疫吸附试验(ELISA)检测细胞因子水平。【结果】HSP患儿MNCs凋亡率明显高于健康对照组患儿,患儿周围血中所测因子水平均高于正常对照组。细胞因子、IGF-1升高的水平与MNCs的凋亡比例成正相关。【结论】HSP患儿MNCs凋亡增加。存在免疫功能紊乱,炎性细胞因子、IGF-1产生过多可能是导致MNCs凋亡增加的重要机制,通过适度调控细胞因子、IGF-1的分泌和MNCs凋亡有可能会改善HSP预后。
【Objective】 To investigate the changes of apoptosis of mononuclear cells (MNCs) in children with henoch-schonlein purpura (HSP) and to detect the changes of cytokines interleukin-8, interleukin-6, Tumor necrosis factor-α and insulin-like growth factor Ⅰ, to explore its impact on the apoptosis of MNCs. 【Methods】 Flow cytometry was used to detect the apoptosis of MNCs in 36 children with HSP and 26 healthy controls. The levels of cytokines were detected by enzyme-linked immunosorbent assay (ELISA). 【Results】 The apoptotic rate of MNCs in children with HSP was significantly higher than that in healthy children. The levels of cytokines in peripheral blood of children with HSP were higher than those in normal controls. The level of cytokines and IGF-1 is positively correlated with the proportion of apoptosis in MNCs. 【Conclusion】 The apoptosis of MNCs in children with HSP increased. The existence of immune dysfunction, inflammatory cytokines, excessive production of IGF-1 may be an important mechanism leading to increased apoptosis of MNCs. HSP may be improved by proper regulation of cytokines, IGF-1 secretion and MNCs apoptosis.