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[目的]探讨胡桃楸的抗肿瘤作用。[方法]通过细胞实验观察胡桃楸提取液对Hela、PC-3细胞增殖的影响;用DNA片断分析靶细胞凋亡情况;通过对端粒酶活性的测定来观察对细胞端粒酶活性的影响。[结果]胡桃楸提取液对肿瘤细胞有明显的细胞毒作用;DNA琼脂糖凝胶电泳可见典型的DNA“梯状条带”;可显著抑制Hela、PC-3细胞端粒酶活性。[结论]胡桃楸具有明显的抗肿瘤作用。
[Objective] To study the anti-tumor effect of Juglans mandshurica. [Method] The effect of Juglans mandshurica extract on the proliferation of Hela and PC-3 cells was observed by cell experiment. The apoptosis of target cells was analyzed by DNA fragment. The telomerase activity was measured by measuring the telomerase activity . [Result] The extract of Juglans mandshurica had obvious cytotoxicity on tumor cells. Typical DNA “ladder-like” bands could be seen on DNA agarose gel electrophoresis, and the telomerase activity of Hela and PC-3 cells was significantly inhibited. [Conclusion] Juglans mandshurica has obvious antitumor effect.