在一些 SLE、急性感染后肾小球肾炎和 Sjg-ren综合征患者的血清中可检测到一种对C3转化酶(C4b2a)具稳定作用的经典补体途径的血清因子,称为C4肾炎因子(C4NeF),它是一种IgG类抗体。为研究SLE患者的B淋巴细胞产生C4NeF的能力,作者从15例SLE患者和10名正常健康人的外周血分离出单个核细胞,在体外用EB病毒感染,以建立有活性的B淋巴细胞系。用EAC3bBb以及EAC14b2a稳定试验测定这些已建立的细胞系上清转化经典和替代途径C3转化酶的能力。结果发 A serum factor of the classical complement pathway stabilizing C3 convertase (C4b2a), known as C4 nephritis, can be detected in sera of patients with glomerulonephritis and Sjögren’s syndrome after some SLE, acute infection Factor (C4NeF), which is an IgG class antibody. To investigate the ability of B lymphocytes to produce C4NeF in SLE patients, we isolated mononuclear cells from peripheral blood of 15 SLE patients and 10 normal healthy individuals and infected them with Epstein-Barr virus in vitro to establish an active B lymphocyte line . The capacity of these established cell line supernatants to transform the canonical and alternative pathway C3 convertases was determined using the EAC3bBb and EAC14b2a stability assays. Results issued