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目的探讨双歧醋对高脂饮食大鼠血脂的影响机制。方法 48只SD大鼠被随机分成双歧醋低剂量组[1.8 mL/(kg.BW)]、中剂量组[3.4 mL/(kg.BW)]、高剂量组[6.8 mL/(kg.BW)]和市售醋组[3.4 mL/(kg.BW)]、高脂模型组以及基础对照组,观察双歧醋对高脂饮食大鼠血脂的影响作用机制,测定指标包括大鼠肝脏脂质、大鼠LDLR的免疫组化分析,HMGCoA还原酶和LDLR的RT-PCR分析。结果双歧醋各组对于HMGCoA还原酶表达有抑制作用,但可以上调LDLR的基因和蛋白质表达。结论双歧醋可以通过调节HMGCoA还原酶和LDLR的表达预防高脂饮食大鼠高血脂的发生。
Objective To investigate the mechanism of bifidone on the lipid profile of rats fed high-fat diet. Methods 48 Sprague-Dawley rats were randomly divided into low-dose bovine vinegar group [1.8 mL / (kg.BW)], middle dose group [3.4 mL / (kg.BW)] and high dose group [6.8 mL / (kg. BW)] and the commercially available vinegar group [3.4 mL / (kg.BW)], high fat model group and basic control group. The mechanism of the effect of bifidame vinegar on blood lipids in high fat diet rats was observed. Lipid, immunohistochemistry of rat LDLR, RT-PCR analysis of HMGCoA reductase and LDLR. Results The bifidal vinegar inhibited the expression of HMGCoA reductase, but increased the expression of LDLR gene and protein. Conclusion Bifidone can prevent hyperlipidemia in high fat diet rats by regulating the expression of HMGCoA reductase and LDLR.