论文部分内容阅读
目的建立一测多评法同时测定芹菜籽中芹菜甲素、芹菜乙素和新蛇床内酯的含量,验证该方法在芹菜籽中应用的准确可行性。方法以芹菜籽原料药中主要成分芹菜甲素、芹菜乙素和新蛇床内酯为指标,在一定范围内建立芹菜甲素与芹菜乙素、芹菜甲素与新蛇床内酯的校正因子。测定芹菜甲素的含量,测得校正因子f芹菜甲素/芹菜乙素=0.226;用校正因子测定芹菜乙素和新蛇床内酯的含量,测得校正因子f芹菜甲素/新蛇床内酯=0.702,同时,采用外标法测定6批不同批次中3个成分的含量,以验证一测多评法的准确性和科学性。结果不同仪器所得相对校正因子的RSD为4.4%～6.7%,同一台仪器不同色谱柱所得相对校正因子的RSD为2.3%～3.6%,建立的校正因子重现性良好,原料药中采用校正因子计算的含量值与外标法实测值之间没有明显差异。结论在缺乏对照品的情况下,该方法可用于芹菜籽中多成分质量评价研究。
OBJECTIVE To establish a multi-evaluation method for simultaneous determination of celerychin, apigenin and new snake bed lactone in celery seed, and to verify the accuracy and feasibility of the method in celery seed. Methods The main components of celery seed raw celery celery a, celestialin B and the new snake bed lactone as an indicator, within a certain range of celery to establish a celery a, a celery a prime and the new snake bed lactone correction factor. Determination of celerythine content, measured the correction factor f celery a prime / apigenin = 0.226; with a calibration factor determination of celery B and lactone lactone lactone content, the calibration factor f celery a / new snake bed Lactone = 0.702. At the same time, the content of three components in six batches of different batches was determined by external standard method to verify the accuracy and scientificity of multiple evaluation method. Results The RSDs of relative calibration factors obtained from different instruments were 4.4% -6.7%. The RSDs of relative calibration factors obtained from different columns of the same instrument ranged from 2.3% to 3.6%. The correction factors established were reproducible. The calibration factors There is no significant difference between the calculated content and the actual value of external standard method. Conclusion In the absence of reference substance, this method can be used to evaluate the quality of celery seed in multiple components.