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目的探讨轴突生长抑制因子少突胶质细胞髓鞘糖蛋白(OMgp)在新生大鼠缺氧缺血性脑损伤(HIBD)后海马区的表达及意义。方法将128只7日龄SD大鼠随机分为HIBD组和对照组各64只。建立HIBD模型,取HIBD后1、3、7天和14天为观察点,采用实时荧光定量聚合酶链反应(RT-PCR)技术和免疫组织化学技术,检测各时间点OMgp在海马区的表达情况(n=8)。结果 RT-PCR结果显示,OMgp mRNA表达从HIBD后1天开始升高,7天达高峰,14天降低,并且HIBD组各个时间点OMgp mRNA表达量均高于对照组[1天:(1.54±0.12)比(1.11±0.19),3天:(2.56±0.14)比(1.14±0.08),7天:(2.95±0.11)比(1.43±0.17),14天:(2.53±0.11)比(1.08±0.08),P<0.05]。免疫组织化学结果显示,HIBD组与对照组比较,海马区OMgp蛋白表达在HIBD后1天开始升高,7天达高峰,14天表达降低,各时间点OMgp蛋白表达量均高于对照组[1天:(33.69±0.75)比(15.67±1.18),3天:(40.89±1.20)比(30.81±2.56),7天:(60.55±1.72)比(48.53±0.65),14天:(51.31±2.06)比(17.40±0.57),P<0.05]。结论 OMgp蛋白可能在未成熟中枢神经系统的发育及损伤中起重要作用。
Objective To investigate the expression and significance of axonal growth inhibitory factor oligodendrocyte myelin glycoprotein (OMgp) in hippocampus of neonatal rats after hypoxic-ischemic brain damage (HIBD). Methods 128 7-day-old SD rats were randomly divided into HIBD group and control group of 64 rats. The HIBD model was established. HIBD was performed at 1, 3, 7 and 14 days after HIBD. The expression of OMgp in hippocampus was detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry Situation (n = 8). Results RT-PCR results showed that the expression of OMgp mRNA increased from the 1st day after HIBD, reached the peak on the 7th day, decreased on the 14th day, and the expression of OMgp mRNA in the HIBD group was higher than that in the control group [1 day: (1.54 ± (1.11 ± 0.19), (2.56 ± 0.14) vs (1.14 ± 0.08), 7 days (2.95 ± 0.11) vs (1.43 ± 0.17), 14 days (2.53 ± 0.11) ± 0.08), P <0.05]. The results of immunohistochemistry showed that the expression of OMgp protein in hippocampus began to increase on the 1st day after HIBD, reached its peak on the 7th day, decreased on the 14th day, and the expression of OMgp protein in the HIBD group was higher than that in the control group (33.69 ± 0.75) vs (15.67 ± 1.18), 3 days: (40.89 ± 1.20) vs (30.81 ± 2.56), 7 days: (60.55 ± 1.72) vs (48.53 ± 0.65), 14 days ± 2.06) than (17.40 ± 0.57), P <0.05]. Conclusion OMgp protein may play an important role in the development and injury of immature central nervous system.