Assessment of tree species diversity, structure and regeneration status of four sacred groves of Kushalnagar,southern Karnataka was carried out. The random quadrat method was employed in each grove for enumeration of tree diversity and regeneration status. A total of 98 tree species belonging to 38 families were found from the sampled areas.These groves possess higher diversity and basal area. Some67 % of species have shown regeneration, while 17 % of species had no regeneration. Another 15 % of species were reappearing or immigrating. It is observed that increased disturbance was directly proportional to number of reappearing or immigrating species and inversely proportional to the diversity in all the sacred groves. Significant impact on diversity, species richness and regeneration status of the flora due to manifold anthropogenic activities have been recorded. Assessment of tree species diversity, structure and regeneration status of four sacred groves of Kushalnagar, southern Karnataka was carried out. The random quadrat method was employed in each grove for enumeration of tree diversity and regeneration status. A total of 98 tree species belonging to 38 families were found from the sampled areas. these groves possess higher diversity and basal area. Some67% of species have shown regeneration, while 17% of species had no regeneration. Another 15% of species were reappearing or immigrating. It is observed that increased disturbance was directly proportional to number of reappearing or immigrating species and inversely proportional to the diversity in all the sacred groves. Significant impact on diversity, species richness and regeneration status of the flora due to manifold anthropogenic activities have been recorded.