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对常规柑桔茎尖嫁接脱毒技术作了改进,将原有5个步骤(砧木种子剥去种皮播于试管培养基—茎尖嫁接—嫁接苗试管培养—转接—转接苗培育)缩减为3个步骤(砧木种子不剥种皮播于培养土—茎尖嫁接—移栽),从而完全省去了试管培养过程。
The technique of grafting and detoxification of conventional citrus shoot tips was improved. The original five steps (seed coat stripped of rootstocks were sown in test tube medium - stem shoot grafting - grafted tube test-tube culture - transfer - seedling cultivation) Reduced to 3 steps (rootstock seed not peeled seedlings in culture soil - shoot tip grafting - transplanting), thus completely eliminating the tube culture process.