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将酿酒葡萄品种白诗南、梅郁的花丝接种在含６ＢＡ２．０ｍｇ·１－１、２，４－Ｄ０．５ｍｇ·１－１的Ｂ５诱导培养基上，诱导产生胚性愈伤组织。胚性愈伤组织经液体悬浮培养形成含大量胚性细胞团的悬浮培养物。用Ｃｅｌｌｕｌａｓｅ Ｒｓ２％、Ｐｅｃｔｏｌｙａｓｅ Ｙ２３０．３％，５ｍｍｏｌ·１－１ＣａＣｌ２·２Ｈ２Ｏ、０．６ｍｏｌ·１－１甘露醇、０．３％葡聚糖硫酸钾、ｐＨ５．６～５．８的酶混合液从胚性细胞团分离得到原生质体。原生质体培养到第５天出现第一次分裂，４０ｄ形成上百个细胞的大细胞团，５０ｄ形成０．５～１．０ｍｍ大小的小愈伤组织。这些小愈伤组织在含６ＢＡ０．５ｍｇ·１－１的Ｂ５分化培养基上分化出胚状体进而形成幼苗，在生根培养基上生根形成再生植株。
Embryogenic callus was induced by inoculation of Fusarium graminearum and Meiyu filaments on B5 induction medium with 6BA2.0mg · 1-1,2,4-D0.5mg · 1-1. Embryogenic callus is cultured in liquid suspension to form a suspension culture containing a large amount of embryogenic cell mass. The enzyme mixture of Cellulase Rs 2%, Pectolyase Y 230.3%, 5 mmol · l-1 CaCl2 · 2H2O, 0.6 mol · l-1 mannitol, 0.3% dextran sulfate, pH 5.6 to 5.8 Protoplasts were isolated from embryogenic cell pellets. The protoplasts were cultured for the first time on day 5, and then formed large cell clusters of hundreds of cells on day 40 and small callus on day 0.5 to 1.0 mm. These small callus differentiated into embryoid body on B5 differentiation medium containing 6BA0.5mg · 1-1 to form seedlings and rooted on rooting medium to form regenerated plants.