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用一张具有 182个 RFL P标记的分子连锁图谱和一套重组自交系 ( RIL)群体 ,对水稻植株主茎总叶片数、叶片生长速率、抽穗期和株高等数量性状进行 QTL区间作图研究 ,定位了影响水稻出叶速率的 8个 QTL ,主茎总叶片数的 2个QTL、抽穗期的 3个 QTL和株高的 4个 QTL。对这些 QTL的遗传效应分析并结合先前用同组合材料研究结果 ,为进一步明确一些 QTL的基因功能提供了有用的信息。如控制水稻主茎总叶片数的一个主效 QTL ,即 QL n3,它同时影响分蘖期出叶速率、抽穗期及株高等数量性状。该位点来自特青的等位基因 ,其加性效应可使水稻在温室冬季短日条件下主茎总叶片数增加1.5叶左右 ,抽穗期延迟 9d,同时具有使分蘖期出叶速率降低 0 .2叶 / 10 d的效应。由于该 QTL位点的基因不受光照长度的影响 ,说明它们有可能是由一个影响水稻基本营养生长期的基因控制或者这些基因紧密连锁 ;而另一个 QTL( QH d8)位点的基因对主茎总叶数、抽穗期和株高的效应似乎受光照长度的影响较大
A QTL interval mapping was performed using a molecular linkage map with 182 RFL P markers and a set of recombinant inbred lines (RIL) population on the total number of leaves, leaf growth rate, heading date and plant height of rice plants Eight QTLs affecting rice leaf rate, two QTLs for total leaf number of main stem, three QTLs for heading date and four QTLs for plant height were studied and mapped. Analysis of the genetic effects of these QTLs combined with previous studies using the same combinatorial material provided useful information for further clarifying the gene function of some QTLs. For example, QL n3, a major QTL controlling the total leaf number of main stems in rice, also affects leaf number, heading date and plant height at tillering stage. The locus was derived from Teqing alleles. The additive effect of rice increased the total number of main stems by about 1.5 leaves in winter and shortened days in winter and 9 days in heading, and at the same time reduced the leaf rate at tillering stage by 0 .2 leaf / 10 d effect. Because the QTL locus genes are not affected by the light length, indicating that they may be controlled by a gene that affects the basic vegetative growth stage of rice or that these genes are closely linked; and the gene of another QTL (QH d8) The effect of total number of leaves, heading date and plant height appears to be strongly influenced by light length