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目的建立实用的实验程序，观察不同功能状态下的精子表面超微结构特点。方法用ＢＷＷ上游法分离活动的人精子：ＢＷＷ体外获能、诱导顶体反应和低渗膨胀试验处理后，将精子贴附于阳离子膜玻片上，叔丁醇冷却干燥后真空铂－碳喷镀复型。复型膜经３％氢氟酸剥脱，次氯酸钠腐蚀，透射电镜观察。结果人精子表面复型图像清晰，立体感强，细节较丰富、清晰，获能精子顶体区呈网络状，顶体反应时可见顶体区大量互相吻合的小泡，经低渗处理的精子顶体区出现类似获能和顶体反应的现象。结论表面复型－透射电镜技术可用于观察精子的不同生理状态引起的表面形貌变化，方法简便，可重复性好，具有良好的应用价值。
Objective To establish a practical experimental program to observe the ultrastructural features of sperm surface under different functional states. Methods Separation of active human spermatozoa by BWW upstream method: In vitro BWW capacitation, induced acrosome reaction and hypotonic swelling test treatment, the sperm attached to the cationic membrane glass, tert-butanol cooling and drying after vacuum platinum-carbon spray Complex type. Duplex membrane stripping 3% hydrofluoric acid, sodium hypochlorite corrosion, transmission electron microscopy. Results The human sperm surface complex image clearly, three-dimensional sense of rich, clear, the ability of the sperm acrosome was network-like, acrosome reaction can be seen in a large number of acrosomes coincide with each other vesicles, hypotonic sperm Acrosomes appear similar to capacitation and acrosome reaction. Conclusion The surface complex-transmission electron microscopy technique can be used to observe the changes of the surface morphology caused by different physiological states of sperm. The method is simple and reproducible, and has good application value.