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目的 构建人KIR2DL4-IgFc段融合蛋白基因真核细胞表达载体。方法 用RT-PCR从孕妇蜕膜组织单个核细胞的总mRNA中逆转录扩增KIR2DL4胞外段cDNA,经Nhe I和Bam HI双酶切后,定向插入真核细胞表达载体CD51negl中,然后经酶切和测序鉴定。结果 限制性内切酶酶切和序列分析表明已成功构建CD51negl-KIR2DL4载体。结论 本研究成功构建KIR2DL4-IgFc融合蛋白真核细胞表达载体,为研究KIR2DL4与其配体之间的关系奠定了基础。
Objective To construct eukaryotic expression vector of human KIR2DL4-IgFc fusion protein gene. Methods The extracellular domain of KIR2DL4 cDNA was reverse transcribed from the total mRNA of decidual tissue mononuclear cells by RT-PCR and inserted into the eukaryotic expression vector CD51negl. Digestion and sequencing identification. Results Restriction endonuclease digestion and sequence analysis showed that CD51negl-KIR2DL4 vector was successfully constructed. Conclusion This study successfully constructed KIR2DL4-IgFc fusion protein eukaryotic expression vector, which laid the foundation for the study of the relationship between KIR2DL4 and its ligand.