论文部分内容阅读
已证明黄曲霉毒素(AFT)可诱发多种动物产生肝癌以及发生急性肝坏死。然而,各种动物对AFT的敏感性是大不相同的。例如小鼠对于AFT的致癌作用具有拮抗性,而大鼠则相当敏感。因此,本文的目的在于研究体外培养的小鼠肝细胞对AFT-B_1的代谢作用,并与大鼠肝细胞进行比较,此外还研究环己烯氧化物(cyclohexene oxide)和二乙基顺丁烯二酸(diethyl maleate)对诱发肝癌的影响。实验所用雄性小鼠的肝细胞培养物,当把它与[~(14)C]-AFT-B_1一起温育10小时后,89%的[~(14)C]-AFT-B_1转变成水溶性的代谢产物,而只有0.77%的AFT-B_1与细胞的大分子共价
Aflatoxin (AFT) has been shown to induce liver cancer in a variety of animals as well as acute liver necrosis. However, the sensitivity of various animals to AFT is quite different. For example, mice are antagonistic to AFT carcinogenesis while rats are rather sensitive. Therefore, the purpose of this paper is to study the metabolism of AFT-B 1 in mouse hepatocytes cultured in vitro and to compare with rat hepatocytes. In addition, the effects of cyclohexene oxide and diethyl cis-butene Effect of diethyl maleate on induced liver cancer. 89% of [~ (14) C] -AFT-B-1 was converted to a water soluble form in a hepatocyte culture of male mice used in the experiment after it was incubated with [~ (14) C] Sex metabolites, whereas only 0.77% of AFT-B_1 is covalently attached to the cellular macromolecules