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目的研究比较成年和新生豚鼠心脏缺血再灌注期间的功能、代谢、形态的不同改变，为心肌保护提供依据。方法建立离体心脏左心做功模型，以ＳｔＴｈｏｍａｓＨｏｓｐｉｔａｌｃａｒｄｉｏｐｌｅｇｉｃｓｏｌｕｔｉｏｎⅡ（ＳｔＴｈｏｍａｓⅡ号液）为心停搏液，测定成年豚鼠（Ａ组）和新生豚鼠（Ｂ组）心脏缺血前、后和再灌注时（或后）动力学，心肌腺苷酸和丙二醛的含量，并行电子显微镜观察。结果Ｂ组再灌注时（或后）的心功能恢复，心肌腺苷酸贮备和超微结构的改善均低于Ａ组（Ｐ＜０．０５），而丙二醛的含量高于Ａ组（Ｐ＜０．０５）。结论以ＳｔＴｈｏｍａｓⅡ号液为心停搏液时，新生豚鼠心肌在缺血再灌注期间受损程度重于成年豚鼠，氧自由基的产生在其中起重要作用。
Objective To compare the changes of function, metabolism and morphology during myocardial ischemia / reperfusion in adult and newborn guinea pigs, and to provide basis for myocardial protection. Methods The left ventricular cardiac function model of isolated heart was established. Cardiac cardioplegia (St Thomas II fluid) was used as cardiac cardioplegia to measure the changes of heart ischemia in adult guinea pigs (A group) and neonatal guinea pigs (B group) ) Kinetics, myocardial adenosine and malondialdehyde content, electron microscopy observations. Results After reperfusion, the cardiac function recovered, the myocardial adenosine acid reserve and the ultrastructure were improved in group B compared with those in group A (P <0.05), while the content of malondialdehyde in group B was higher than that in group A P <0.05). Conclusion When StThomas Ⅱ fluid is cardioplegic solution, the myocardial damage of neonatal guinea pigs during ischemia reperfusion is more serious than that of adult guinea pigs. The production of oxygen free radicals plays an important role.