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目的:探讨经不同径路将腺病毒导入豚鼠耳蜗后,被感染耳蜗细胞的分布情况。方法:使用构建有EGFP指示基因的腺病毒分别通过圆窗入路进入外淋巴系统,耳蜗侧壁钻孔中阶入路进入内淋巴系统,耳蜗冷冻切片和组织铺片观察腺病毒感染耳蜗细胞的分布情况。结果:由圆窗入路进入外淋巴系统的腺病毒可以感染血管纹的Ⅰ型、Ⅳ型和Ⅴ型纤维细胞、螺旋唇上细胞、前庭膜细胞、Ronsensal孔内的螺旋神经元、前庭阶和鼓阶的上皮细胞,内外毛细胞和支持细胞不被感染;而耳蜗侧壁钻孔中阶入路进入内淋巴系统的腺病毒可以感染听器的支持细胞和血管纹缘细胞等。结论:腺病毒是一种有效的豚鼠耳蜗细胞转染载体,导入耳蜗后,可以将目的基因转染到耳蜗细胞内。通过不同径路将腺病毒导入外、内淋巴系统,耳蜗被感染的细胞范围不一致。仅注入外淋巴系统的腺病毒无法感染内淋巴系统内的细胞,只有将病毒导入内淋巴系统,耳蜗支持细胞才被感染。
Objective: To investigate the distribution of infected cochlear cells after introducing adenovirus into guinea pig cochlea via different pathways. Methods: Adenoviruses harboring EGFP indicator gene were used to enter into the perilymph system through the round window, and the mesangial wall of the cochlear wall was infiltrated into the endolymphatic system. Cochlear cryosections and tissue explants were used to observe the distribution of adenovirus infected cochlear cells . Results: The adenovirus entering the perilymph system by round window could infect the vascular patterns of type I, IV and V fibroblasts, spiral lip cells, vestibular membrane cells, spiral neurons in Ronsensal pore, vestibular and Drum epithelial cells, internal and external hair cells and supporting cells are not infected; and cochlear sidewall drilling into the endolymphatic system into the adenovirus can support the hearing cell support cells and vascular border cells. Conclusion: Adenovirus is an effective transfection vector for cochlear cells of guinea pigs. After being introduced into the cochlea, the target gene can be transfected into cochlear cells. Through different pathways to introduce adenovirus, the endolymphatic system, the cochlear infected cell range is inconsistent. Adenovirus injected only into the lymphatic system can not infect the cells within the endolymphatic system, and only when the virus is introduced into the endolymphatic system, the cochlear supporting cells are infected.