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探讨不同ω-3/ω-6构成比的配伍红花籽油(Compatibility Safflower Seed Oil,CSSO)预防神经细胞氧化损伤的作用。通过过氧化氢(hydrogen peroxide,H2O2)氧自由基供体诱导,建立人神经母细胞瘤SH-SY5Y细胞氧化损伤模型;以不同浓度和ω-3/ω-6构成比的CSSO进行细胞药物干预,利用四甲基偶氮唑蓝(methyl thiazolyltetrazolium,MTT)和流式细胞仪检测细胞活力变化和细胞凋亡率。我们建立了H2O2诱导的SH-SY5Y细胞氧化损伤模型,其IC50值为1089.54μmol/L H2O2;随着ω-3相对含量递减,CSSO预防细胞氧化损伤的效应增加,且当ω-3/ω-6比例为1∶6.68和有效浓度范围为375～750μg CSSO/mL时,其药物干预组细胞活力(84.1%)显著高于模型组(61.1%),而药物干预组细胞凋亡率(12.6%)明显低于模型组(25.9%)。从以上结果可以推测,CSSO能够保护细胞并预防氧自由基诱导的细胞损伤,其效果可能与CSSO中ω-3/ω-6构成比密切相关。
To explore the effect of different ω-3/ω-6 composition ratio of Compatibility Safflower Seed Oil (CSSO) on preventing oxidative damage of nerve cells. The oxidative damage model of human neuroblastoma SH-SY5Y cells was established by the hydrogen peroxide (H2O2) oxygen radical donor induction; cellular drug intervention was performed with CSSO with different concentrations and ω-3/ω-6 ratio. The use of methyl thiazolyltetrazolium (MTT) and flow cytometry to detect cell viability and cell apoptosis. We established an H2O2-induced oxidative damage model of SH-SY5Y cells with an IC50 value of 1089.54 μmol/L H2O2; as the relative amount of ω-3 decreases, the effect of CSSO in preventing oxidative damage to cells increases, and when ω-3/ω- With a ratio of 1:6.68 and an effective concentration range of 375-750 μg CSSO/mL, the cell viability (84.1%) in the drug intervention group was significantly higher than that in the model group (61.1%), while the apoptosis rate in the drug intervention group (12.6%). ) was significantly lower than the model group (25.9%). From the above results, we can speculate that CSSO can protect cells and prevent oxygen free radical-induced cell damage, and its effect may be closely related to the composition ratio of omega-3/ω-6 in CSSO.