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目的:观察大建中汤对粘连性肠梗阻大鼠肠黏膜免疫系统中CD4+、CD8+T淋巴细胞及SIgA表达的影响。方法:复制粘连性肠梗阻大鼠模型,将140只SD大鼠随机分7组,即正常组、模型组、假手术组、理中组、建大组、建中组、建小组,分别予生理盐水、附子理中丸、大建中汤灌胃治疗。于造模术后7、14 d各取一半大鼠取回肠组织,运用免疫组织化学染色法标记小肠黏膜中CD4+、CD8+T淋巴细胞及SIgA数量,用图像分析仪统计其含量。结果:与正常组比较,模型组大鼠肠黏膜免疫系统中SIgA及CD4+、CD8+T淋巴细胞数量明显减少,差异具有统计学意义(P<0.05);建大组较模型组上述各指标均有升高(P<0.05),其中CD4+、CD8+T淋巴细胞数量显著升高(P<0.01);各给药组治疗7 d与14 d后无明显差异。结论:大建中汤可增强粘连性肠梗阻大鼠肠黏膜免疫功能,发挥肠黏膜的保护作用。
Objective: To observe the effect of Dajianzhong Decoction on the expression of CD4 +, CD8 + T lymphocytes and SIgA in the intestinal mucosal immune system in rats with adhesive intestinal obstruction. METHODS: One hundred and forty SD rats were randomly divided into seven groups: normal group, model group, sham operation group, LiZhong group, JianDa group, Jianzhong group and JianGroup, respectively Physiological saline, aconite in the pill, Dajianzhong decoction treatment. The rats were sacrificed on the 7th and 14th day after operation to collect the intestinal tissues. The numbers of CD4 +, CD8 + T lymphocytes and SIgA in the small intestine mucosa were detected by immunohistochemical staining. The content of SIgA was measured by image analyzer. Results: Compared with the normal group, the numbers of SIgA, CD4 + and CD8 + T lymphocytes in the intestinal mucosal immune system of the model group were significantly decreased (P <0.05), and those in the model group were significantly higher than those in the model group (P <0.05). The numbers of CD4 + and CD8 + T lymphocytes were significantly increased (P <0.01). There was no significant difference between the treatment groups on the 7th and 14th days after treatment. Conclusion: Dajianzhong Decoction can enhance intestinal mucosal immune function and promote intestinal mucosal protective effect in rats with adhesive intestinal obstruction.