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目的 探索肝硬化时肝组织细胞胰岛素受体（IR）和酪氨酸蛋白激酶（TPK）含量的变化规律。 方法 应用图象分析系统对12例血清HBV标志物阳性的肝炎后肝硬化患者肝组织石腊包埋切片对抗胰岛素受体和酪氨酸蛋白激酶抗体免疫组化标记物的定量测定。 结果 肝硬化糖耐量试验（GTT）异常组和GTT正常组IR含量分别为（41.44±6.47）AU和(43.36±4.78）AU，较对照组［(48.11±4.42）AU］显著减少（P＜0.01），肝硬化GTT异常组和GTT正常组IR含量无显著性差异（P＞0.05）；TPK表达量：肝硬化GTT异常组较GTT正常组显著性减少，分别为(41.31±5.55）AU和（51.16±5.83）AU。 结论 肝硬化时肝细胞IR减少；肝硬化时GTT异常与肝细胞胰岛素受体酪氨酸蛋白激酶活性减退有关。
Objective To investigate the changes of insulin receptor (IR) and tyrosine protein kinase (TPK) in hepatic tissue of patients with cirrhosis. Methods Quantitative analysis of immunohistochemical markers of insulin receptor and tyrosine kinase antibody in paraffin section of liver tissue of 12 patients with post-hepatitis B cirrhosis with serum HBV markers by image analysis system. Results The levels of IR in GTT group and GTT group were (41.44 ± 6.47) AU and (43.36 ± 4.78) AU, respectively, which were significantly lower than those in control group [(48.11 ± 4.42) AU] (P0.01 (P> 0.05). The level of TPK expression in liver cirrhosis GTT group was significantly lower than that in GTT normal group (41.31 ± 5.55) AU and ( 51.16 ± 5.83) AU. Conclusions Liver cirrhosis reduces the IR of liver cells. GTT abnormalities in liver cirrhosis are related to the decrease of insulin receptor tyrosine protein kinase activity in liver cells.