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采用平板稀释涂布法从泥河湾藉箕滩遗址土壤中分离微生物,结合菌株形态特征、生理生化特征及分子鉴定方法对分离到的微生物进行鉴定,以确定泥河湾藉箕滩遗址不同深度土层中微生物物种分布,为详细了解遗址土壤微生物物种多样性提供依据.结果表明:共分离鉴定到96株细菌、34株放线菌、15株真菌.根据同源性比对及构建系统发育进化树,确定细菌归属于芽孢杆菌属和短波单胞菌属;放线菌归属链霉菌属;真菌归属青霉属、曲霉属、被孢霉属和盐单胞菌藻.NHWB 1047,NHWA 1017,NHWF 1002等菌株与已知菌种相似度低于97%,可能为潜在新种.本研究首次从泥河湾藉箕滩遗址不同深度土层中分离鉴定微生物,是泥河湾考古研究的重要组成部分,为进一步了解史前时代生态环境提供了科学依据.
Micro-organisms were isolated from the soil of the site of Leijian Beach in Nihewan by using the plate dilution coating method. The isolated microorganisms were identified by morphological, physiological and biochemical characteristics and molecular identification methods to determine the depth The results showed that 96 strains of bacteria, 34 strains of actinomycetes and 15 strains of fungi were isolated and identified.According to homology comparison and phylogeny The phylogenetic tree was identified as belonging to the genus Bacillus and Brevundus spp .; Actinomyces belonged to Streptomyces; fungi belonged to Penicillium, Aspergillus, Mortierella and Salmonella. NHWB 1047, NHWA 1017 , NHWF 1002 and other strains with known strains less than 97% may be potential new species.This study for the first time from the depth of the soil in the depth of Leijimen site of the Nihe Bay microbial identification, is the study of the An important component for providing a scientific basis for further understanding of the ecological environment in prehistoric times.