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基于激光诱导荧光的测量原理,研制了一种用于心肌钙蛋白Ⅰ(cTnⅠ)快速检测的便携式系统。首先在测试杯中cTnⅠ与荧光试剂发生免疫反应,经过分离除去多余的荧光试剂,测试杯中的荧光分子与cTnI的量成相关性,通过对荧光分子进行诱导激发,使其发出荧光信号,然后经过光电转换、微弱电信号放大、信号采集与处理,最终实现对cTnⅠ的快速定量检测。临床实验显示,在cTnT为1.5~12.2 ng/mL范围内,本系统与TosohAIA-360生化分析仪的检测结果具有很好的线性关系,样品数为6时两者的相关系数达到0.97。
Based on the principle of laser-induced fluorescence measurement, a portable system for the rapid detection of cardiac troponin I (cTnI) was developed. First in the test cup cTnI and fluorescence reagent immune reaction, after removal of excess fluorescent reagent, the test cup fluorescence molecules and cTnI volume is related to the fluorescence molecules by inducing excitation, so that it emits a fluorescence signal, and then After photoelectric conversion, weak signal amplification, signal acquisition and processing, the final realization of cTn Ⅰ rapid quantitative detection. Clinical experiments showed that there was a good linear relationship between the system and the TosohAIA-360 biochemical analyzer when the cTnT was 1.5-12.2 ng / mL. The correlation coefficient between the two systems was 0.97 when the sample number was 6.