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土壤盐碱化是影响玉米产量的重要非生物因素之一。用200 mmol/L NaCl溶液对耐盐玉米自交系E28进行盐处理,提取叶片可溶性蛋白质进行双向电泳,并用ImageMasterTM2D 6.00软件分析,探究玉米的耐盐机理。结果表明,盐胁迫处理后共有10个有明显差异的蛋白点,其中4个表达上调,6个表达下调。质谱分析和数据库搜索鉴定出应激反应蛋白和类囊体腔19 KDa蛋白,可能是盐胁迫后植物营养生长及光合作用受到影响的结果。
Soil salinization is one of the most important abiotic factors affecting maize yield. The salt-tolerant maize inbred line E28 was treated with 200 mmol / L NaCl solution. The soluble protein was extracted by two-dimensional gel electrophoresis and analyzed by ImageMasterTM 2D 6.00 software to explore the salt tolerance mechanism of maize. The results showed that there were 10 protein spots with significant difference after salt stress treatment, of which 4 were up-regulated and 6 down-regulated. Mass spectrometry and database searches identified 19 KDa proteins in stress response proteins and thylakoid cavities as a result of vegetative growth and photosynthesis after salt stress.