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本文对人外周血单个核细胞(PBMC)诱生白细胞介素2(IL-2)及用ConA 激活的小鼠脾细胞检测IL-2活性过程中的几个实验条件进行了摸索,发现1×10~6 PBMC/ml 经10ug ConA/ml 刺激48h 为诱生IL-2的最佳条件,检测IL-2活性的最佳条件是:2.5×10~4每孔检测细胞/0.1ml/与等量样品共同培养24h.作者认为,小鼠脾细胞经10ug ConA/ml 剌激48h 后.大部分转化为淋巴母细胞,适用于IL-2活性的检测,并具有一定的特异性和重复性。
In this paper, several experimental conditions of interleukin 2 (IL-2) induced by human peripheral blood mononuclear cells (PBMCs) and the activity of IL-2 detected by ConA-activated mouse spleen cells were explored and found that 1 × The optimum conditions for inducing IL-2 were as follows: 10 ~ 6 PBMC / ml stimulated IL-2 by 10ug ConA / ml for 48h, the optimum conditions for the detection of IL-2 activity were: 2.5 × 10 ~ 4 cells / 0.1ml / The amount of sample co-cultured 24h.The author believes that mouse spleen cells stimulated by 10ug ConA / ml after 48h, most of the transformed into lymphoblasts, IL-2 activity for the detection, and has a certain specificity and repeatability.