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目的 :探讨千佛菌对肾阳虚小鼠Mф -IL-1-Th1免疫调节网络的影响。方法 :将BALB/c小鼠随机分为 4组 ,每组 10只 ,除空白对照组外 ,其余三组肌注HCS制备肾阳虚模型。然后其中两组模型小鼠分别用千佛菌 2 0 0 % (W/V)、5 0 % (W /V)浓度煎液 0 .4ml/只·d× 14d灌胃。采用吞噬中性红比色法、MTT比色法分别测定Mф、IL-1、Th1的含量或活性。结果 :肾阳虚模型小鼠与正常对照组相比 ,腹腔Mф吞噬功能、IL-1含量、Th1活性均明显降低 (P <0 .0 1) ,用千佛菌灌胃后 ,Mф吞噬功能、IL-1含量及Th1活性均明显升高 (P <0 .0 5 ,P <0 .0 1)。结论 :千佛菌对肾阳虚小鼠Mф -IL-1-Th1免疫网络有正向调节作用。
Objective :To investigate the effect of Thymus spp.on the immune regulatory network of Mф-IL-1-Th1 in mice with kidney-yang deficiency. METHODS: BALB/c mice were randomly divided into 4 groups, 10 in each group. Except the blank control group, the remaining three groups were injected intramuscularly with HCS to prepare the model of kidney-yang deficiency. Then, two groups of model mice were intragastrically administered with 200 mL (W/V) and 50 % (W/V) decoction at a concentration of 0.4 ml/dx 14 d. The phagocytic neutral red colorimetric method and MTT colorimetric method were used to determine the content or activity of Mф, IL-1, and Th1, respectively. RESULTS: Compared with the normal control group, the kidney yang deficiency model mice had significantly decreased peritoneal M phagocytosis, IL-1 content, and Th1 activity (P < 0.01). After swallowing with thousands of fungus, M phagocytosis was observed. , IL-1 content and Th1 activity were significantly increased (P <0. 05, P <0. 01). Conclusion : Thousand Buddha fungus positively regulates the immune network of Mф-IL-1-Th1 in mice with kidney-yang deficiency.