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目的超声引导下95%医用酒精硬化治疗卵巢巧克力囊肿(巧囊)前后血清IL-6、JunB、beta-1、4-GalT-Ⅰ的表达差异。方法选取超声引导下酒精硬化治疗的9例巧囊患者,术前、后3个月肘静脉采血标本,采用NimbleGen全基因组表达谱芯片法检测,筛选出治疗前、后差异表达的显著因子,并分析有显著表达差异因子的功能及通路与巧囊介入治疗的相关性。结果在NimbleGen全基因组表达谱芯片检测中筛选出IL-6、JunB及beta-1、4-GalT-Ⅰ;酒精硬化治疗巧囊术可使这3条因子表达下调,它们参与的免疫调节、血管形成、细胞粘连等功能与巧囊的发病机制具有相关性,并通过7条信号通路发挥作用。结论巧囊患者血清IL-6、JunB及beta-1,4-GalT-Ⅰ在酒精硬化治疗后呈下调表达,具有从分子水平评价介入治疗疗效的价值。
Objective To investigate the difference of serum IL-6, JunB, beta-1, 4-GalT-Ⅰ levels before and after treatment with 95% alcoholic sclerosis on ovarian chocolate cysts. Methods 9 cases of clever cystic patients undergoing ultrasound guided alcohol sclerotherapy were selected. Blood samples were collected from the elbow venous before and 3 months after operation. NimbleGen whole genome cDNA microarray was used to screen the significant differentially expressed genes Analysis of significant differences in the expression of the function of factors and pathways involved with the intervention of clever capsule. RESULTS: IL-6, JunB and beta-1,4-GalT-I were screened by NimbleGen genome-wide expression microarray. Alcohol sclerotherapy and cystic surgery could down-regulate the expression of these three factors and participate in the immunomodulation and vascular Formation, cell adhesion and other functions with the pathogenesis of Qiao capsule has relevance, and through the seven signaling pathway play a role. Conclusion The serum levels of IL-6, JunB and beta-1,4-GalT-I in patients with Qiao capsule are down-regulated after alcoholic sclerosis treatment, which has the value of evaluating the efficacy of interventional therapy at the molecular level.