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目的探讨冠心病患者外周血内皮祖细胞(EPCs)的水平及其对内皮功能的影响。方法随机选取经冠脉造影确诊为冠心病患者53例为冠心病组,非冠心病患者52例为正常对照组,采用超声测定其内皮舒张功能指标(FMD),采用流式细胞术检测各组EPCs水平,同时检测反映内皮功能的生化指标,采用免疫散射比浊法测定高敏C反应蛋白(hs-CRP)水平,硝酸盐还原酶法测定NO。结果在基线水平上,冠心病组内皮功能较正常对照组明显低下,EPCs数目明显减少,hs-CRP水平升高,NO含量减低,差异均有统计学意义(P均<0.05);FMD与EPCs数量显著相关,FMD随EPCs数量增加而改善,呈正相关趋势(r=0.52,P<0.01)。同时,反映内皮功能的指标hs-CRP与FMD、EPCs均呈负相关趋势(r=-0.43,r=-0.50,P<0.05),NO与FMD、EPCs均呈正相关趋势(r=0.54,r=0.45,P<0.05)。结论冠心病患者EPCs数量较健康人群明显减少,内皮功能明显低下,同时内皮功能随EPCs数量增加而改善。
Objective To investigate the levels of endothelial progenitor cells (EPCs) and their effect on endothelial function in patients with coronary heart disease. Methods Fifty-three patients with coronary heart disease diagnosed by coronary angiography were randomly divided into coronary heart disease group and 52 non-coronary heart disease patients as normal control group. The endothelium-dependent vasodilatation index (FMD) was measured by ultrasonography. Flow cytometry EPCs were measured. The biochemical indexes reflecting the function of endothelial cells were also detected. The levels of hs-CRP were determined by immune nephelometry. NO was determined by nitrate reductase method. Results At baseline, the endothelial function in CHD group was significantly lower than that in the normal control group, the number of EPCs was significantly decreased, the level of hs-CRP was increased and the content of NO was decreased (all P <0.05) There was a significant positive correlation between FMD and the number of EPCs (r = 0.52, P <0.01). At the same time, hs-CRP, a marker of endothelial function, showed negative correlation with FMD and EPCs (r = -0.43, r = -0.50, P <0.05) = 0.45, P <0.05). Conclusion The number of EPCs in patients with coronary heart disease was significantly lower than that in healthy people, the endothelial function was significantly lower, and endothelial function improved with the increase of EPCs.