论文部分内容阅读
目的研究志贺菌属中非典型1类整合子的分布及其与耐多药性的关系。方法对实验室保存的90株志贺菌属进行8种抗生素药敏实验,针对非典型1类整合子的耐药基因盒区域设计引物进行聚合酶链反应(PCR)扩增和限制性片段长度多态性(RFLP)分析,对代表性菌株进行测序分析。结果 90株志贺菌属中有86株(95.6%)对≥3种抗生素耐药;有66株(73.3%)对氨苄青霉素(AMP)、四环素(TET)和氯霉素(CHL)联合耐药;非典型1类整合子分布于65株(72.2%)志贺菌属中;RFLP分析结果表明,所有酶切产物的带型均一,测序分析发现序列为blaoxa-30-aadA1;非典型1类整合子阳性菌株对AMP-TET-CHL联合耐药率(81.5%)高于阴性菌株(52.0%),经双侧χ2检验发现差异有统计学意义(P<0.05)。结论志贺菌属非典型1类整合子阳性与其对AMP-TET-CHL联合耐药有关,可能参与耐多药性的形成。
Objective To study the distribution of the type 1 integrins of Shigella and their relationship with multidrug resistance. Methods Eight antibiotics susceptibility tests were carried out on 90 strains of Shigella preserved in the laboratory. Primers were designed for polymerase chain reaction (PCR) amplification and restriction fragment length Polymorphism (RFLP) analysis was performed on representative strains for sequencing analysis. RESULTS: Of the 90 strains of Shigella, 86 (95.6%) were resistant to ≥3 antibiotics; 66 strains (73.3%) were resistant to ampicillin (AMP), tetracycline (TET) and chloramphenicol The atypical class 1 integron was distributed in 65 strains (72.2%) of Shigella. The results of RFLP analysis showed that the banding patterns of all the digested products were homogeneous and the sequence was blaoxa-30-aadA1. The atypical 1 The positive rate of combined integron positive strains to AMP-TET-CHL was higher than that of negative strains (81.5%) (52.0%). There was significant difference (P <0.05) by two-sided χ2 test. Conclusions The positive rate of atypical class 1 integron of Shigella is related to its resistance to AMP-TET-CHL, which may be involved in the formation of multi-drug resistance.