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目的 研究金地鼠颊囊癌细胞增殖与细胞凋亡的改变。方法 建立二甲基苯并蒽 (DMBA)诱导金地鼠颊囊癌模型 ,流式细胞仪 (FCM )检测细胞DNA倍体、S期细胞比例 (SPF)及细胞凋亡 (Apoptosis ,Apop)的变化。结果 颊囊癌的异倍体检出率较正常颊囊黏膜高。原位癌时SPF与Apop较正常颊囊黏膜增高。Ⅰ级鳞癌时 ,SPF较原位癌时不但未增高反而下降 (P <0 .0 5 ) ,而Apop较原位癌下降 (P <0 .0 5 )。Ⅱ级鳞癌时 ,SPF较Ⅰ级鳞癌时增高 (P <0 .0 5 ) ,与原位癌比较无显著性差异 (P >0 .0 5 ) ;而Apop较原位癌时下降 (P <0 .0 5 ) ,与Ⅰ级鳞癌比较无显著性差异 (P >0 .0 5 )。结论 本实验结果提示口腔癌的发生与发展和细胞增殖与细胞凋亡的关系失调有关。它们的进一步研究将有助于了解口腔癌的生物学特性 ,有助于其病理诊断 ,估计预后 ,指导治疗
Objective To study the changes of proliferation and apoptosis of Hamster cheek pouch cancer cells. Methods DMBA-induced hamster cheek pouch carcinoma model was established. The changes of DNA ploidy, S phase fraction and apoptotic cells (Apoptosis, Apoptosis) were detected by flow cytometry (FCM) . Results The aneuploidy rate of cheek pouch was higher than that of normal cheek pouch. In situ carcinoma SPF and Apop than the normal cheek pouch mucosa increased. In grade Ⅰ squamous cell carcinoma, SPF was not increased but decreased (P <0.05), while Apop was lower than that of carcinoma in situ (P <0.05). (P <0.05), SPF was significantly higher than that of grade I squamous cell carcinoma (P <0.05), but not significantly different from that of carcinoma in situ (P> 0.05) P <0.05), and grade Ⅰ squamous cell carcinoma no significant difference (P> 0.05). Conclusion Our results suggest that the occurrence and development of oral cancer and cell proliferation and apoptosis related to the imbalance. Their further research will help to understand the biological characteristics of oral cancer, contribute to their pathological diagnosis, prognosis, guide treatment