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目的 COX-2的过度表达在肿瘤的发生发展中起重要作用,本文探讨COX-2启动子区单核苷酸多态与胃癌易感性的关系。方法以聚合酶链反应-限制性片段长度多态性对155例胃癌患者和237例正常对照进行基因分型,以logistic回归的比值比(OR)及其置信区间(CI)来评估风险度。结果 COX-2-1290AG及GG基因型与胃癌的发病风险无关,OR为1.24(95%CI=0.66-2.35)。而携带COX-2-1195GA或-1195AA基因型的个体胃癌发病风险显著增加,OR分别为1.91(95%CI=1.05-3.47),2.71(95%CI=1.40-5.27)。单体型分析发现A_1290-A_1195单体型显著增加了胃癌的发病风险(OR=1.49,95%CI=1.10-2.01)。结论 COX-2启动子区-1195G→A遗传变异与胃癌发病风险相关。
Objective COX-2 overexpression plays an important role in tumorigenesis and development. This article explored the relationship between COX-2 promoter SNP and gastric cancer susceptibility. Methods The genotypes of 155 patients with gastric cancer and 237 healthy controls were genotyped by polymerase chain reaction - restriction fragment length polymorphism. The odds ratio (OR) and confidence interval (CI) of logistic regression were used to evaluate the risk. Results The COX-2-1290AG and GG genotypes were not associated with the risk of gastric cancer. The OR was 1.24 (95% CI = 0.66-2.35). However, the risk of developing gastric cancer was significantly increased in individuals with COX-2-1195GA or -1195AA genotypes, with ORs of 1.91 (95% CI = 1.05-3.47) and 2.71 (95% CI = 1.40-5.27), respectively. Haplotype analysis found that A_1290-A_1195 haplotype significantly increased the risk of gastric cancer (OR = 1.49, 95% CI = 1.10-2.01). Conclusion The genetic variation of -1195G → A in COX-2 promoter region is associated with the risk of gastric cancer.