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目的探讨肿瘤干细胞标志物CD133、ABCG2及p75NTR在非小细胞肺癌(NSCLC)组织中的表达及其生物学意义。方法采用免疫组织化学方法,检测44例NSCLC组织、17例癌旁组织、17例非肿瘤组织中的CD133、ABCG2及p75NTR蛋白的表达并对结果进行分析。结果 (1)CD133、ABCG2及p75NTR蛋白在44例NSCLC组织中的阳性表达率分别为68.2%(30/44)、31.8%(14/44)、47.7%(21/44),在17例癌旁组织中分别为5.9%(1/17)、0(0/17)、11.8%(2/17),在17例非肿瘤组织中分别为47.1%(8/17)、11.8%(2/17)、17.6%(3/17),差异均有统计学意义(P<0.05);其中NSCLC组织中p75NTR蛋白的阳性表达与肿瘤的远处转移密切相关(P<0.05);(2)随肿瘤恶性度的增加,CD133蛋白的阳性表达率也明显增加,差异接近统计学意义(P=0.07)。结论 NSCLC组织中CD133、ABCG2及p75NTR的表达明显高于癌旁及非肿瘤组织,或许这三者与NSCLC恶性演进有关;肿瘤干细胞标记物CD133的表达与NSCLC的分化密切相关,CD133蛋白或许能成为NSCLC的肿瘤干细胞标记物。
Objective To investigate the expression of tumor stem cell markers CD133, ABCG2 and p75NTR in non-small cell lung cancer (NSCLC) and its biological significance. Methods The expression of CD133, ABCG2 and p75NTR in 44 NSCLC tissues, 17 adjacent non-cancerous tissues and 17 non-neoplastic tissues were detected by immunohistochemistry and the results were analyzed. Results (1) The positive rates of CD133, ABCG2 and p75NTR in 44 NSCLC tissues were 68.2% (30/44), 31.8% (14/44) and 47.7% (21/44) respectively, There were 5.9% (1/17), 0 (0/17) and 11.8% (2/17) in the adjacent tissues and 47.1% (8/17) and 11.8% (2/17) in 17 non-tumor tissues, (P <0.05). The positive expression of p75NTR protein in NSCLC was closely related to the distant metastasis of tumor (P <0.05); (2) The positive rate of CD133 protein increased with the increase of malignancy, the difference was close to statistical significance (P = 0.07). Conclusions The expression of CD133, ABCG2 and p75NTR in NSCLC tissues is significantly higher than that in adjacent non-cancerous tissues. These three may be related to the malignant progression of NSCLC. The expression of CD133, a tumor stem cell marker, is closely related to the differentiation of NSCLC. CD133 protein may become NSCLC Of cancer stem cell markers.