【摘 要】
：
目的观察以纤维蛋白胶(fibrinsealant,FS)为载体的注射型骨修复材料对体外培养的兔骨髓基质细胞(marrowstromalcell,MSC)增殖和超微结构的影响.方法实验分组为实验组(FS+bFGF
【机 构】
：
解放军第四军医大学西京医院,陕西,西安,710033石家庄军械工程学院门诊部,河北,石家庄,050000;
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目的观察以纤维蛋白胶(fibrinsealant,FS)为载体的注射型骨修复材料对体外培养的兔骨髓基质细胞(marrowstromalcell,MSC)增殖和超微结构的影响.方法实验分组为实验组(FS+bFGF+rhBMP2)、对照组1(FS)、对照组2(FS+bFGF)、对照组3(FS+rhBMP2)及单纯对照组.采用细胞培养、MTT及电镜等方法对各组兔MSC的增殖及超微结构进行研究.结果(1)各组材料对细胞促增殖作用由强到弱依次是:对照组2(FS+bFGF)、实验组(FS+bFGF+rhBMP2)、对照组1(FS)、对照组3(FS+rhBMP2)、单纯对照组,差异有显著性(P<0.05);(2)扫描电镜观察发现,材料表面粗糙,有微孔存在,各组细胞与材料融合生长.透射电镜观察发现:实验组的绝大部分细胞呈成骨细胞表型,细胞增殖状态旺盛,分化好.各对照组细胞或是细胞增殖活性高,但向成骨细胞表型分化差,或是向成骨细胞表型分化好,但细胞增殖活性较差.结论FS+bFGF+rhBMP2即可显著促进MSC增殖和兔MSC向成骨细胞方向的分化水平.
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