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目的 为探讨生长抑素 (SOM)在癫痫发病中的作用。方法 应用原位杂交组织化学方法研究慢性癫痫大鼠海马回、齿状回、大脑皮质 SOM m RNA表达的变化 ,并用放射免疫法检测了海马、大脑皮质内基因表达产物的变化。结果 慢性癫痫大鼠海马回、齿状回、大脑皮质 SOM m RNA胞体数量、胞体截面积均明显高于对照组 ,胞体灰度值均明显低于对照组。与此同时 ,海马及大脑皮质内 SOM含量亦明显增高。结论 慢性癫痫发病过程中编码 SOM的基因被活化 ,同时伴随着其表达产物的增加 ,SOM与癫痫的发病密切相关。
Objective To investigate the role of somatostatin (SOM) in the pathogenesis of epilepsy. Methods The expression of SOM m RNA in hippocampal gyrus, dentate gyrus and cerebral cortex in rats with chronic epilepsy was detected by in situ hybridization histochemistry. The changes of gene expression in hippocampus and cerebral cortex were detected by radioimmunoassay. Results The number of SOM m RNA cytoplasm and cell body in the hippocampus gyrus, dentate gyrus and cerebral cortex of chronic epileptic rats were significantly higher than that of the control group, and the gray value of the cell bodies was significantly lower than that of the control group. At the same time, SOM levels in the hippocampus and cerebral cortex also significantly increased. Conclusion The gene encoding SOM is activated in the pathogenesis of chronic epilepsy, accompanied by the increase of its expression product, SOM is closely related to the onset of epilepsy.