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目的:对中药材远志及其混伪品进行分子鉴定,保障药材质量及用药安全。方法:对46份样品进行DNA提取,通过聚合酶链式反应(PCR)扩增其psbA-trnH序列并双向测序,应用CodonCode Aligner V3.7.1对测序峰图校对拼接,去除低质量区和引物区,得到psbA-trnH序列,用MEGA 6.0对序列进行比对分析,基于K2P遗传距离构建系统聚类树。结果:通过相似性搜索法能准确鉴别远志及其混伪品,远志两个基原的种内最大K2P距离分别为0.004和0,均小于其与混伪品的种间最小K2P遗传距离(0.010和0.005),基于psbA-trnH序列的系统发育树可将远志药材及其混伪品明显区分开。结论:psbA-trnH序列能有效鉴别远志药材及其混伪品,为中药材的质量控制提供新方法。
OBJECTIVE: To molecularly identify Polygalaceae and its adulterants, and to ensure the quality and safety of medicinal materials. Methods: DNA samples were extracted from 46 samples. The psbA-trnH sequence was amplified by polymerase chain reaction (PCR) and sequenced. CodonCode Aligner V3.7.1 was used to calibrate the sequencing peak map, and the low quality and primer regions were removed The sequence of psbA-trnH was obtained. The sequence was analyzed by MEGA 6.0, and the phylogenetic tree was constructed based on the genetic distance of K2P. Results: The similarity search method can be used to identify Polygalaceae and its adulterated products. The maximum K2P distances of the two primordial species of Polygalaceae were 0.004 and 0, respectively, which were less than the minimum K2P genetic distances (0.010 And 0.005). Phylogenetic trees based on the psbA-trnH sequence clearly distinguished Polygalaceae and its adulterants. Conclusion: psbA-trnH sequence can effectively identify Polygalaceae and its adulterants, providing a new method for the quality control of Chinese herbal medicines.