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目的:对本地区RhD阴性患者的DEL型分布情况进行调查分析。方法:采用Rh血型抗原检测卡对患者进行RhD初筛,改良间接抗球蛋白试验(IAT)进行RhD阴性确认,吸收放散-微柱凝胶法检测DEL型,采用单克隆的IgM类C、c、E、e抗血清进行RhCE表型的检测。结果:初筛和IAT确认为RhD阴性的192例标本中,吸收放散-微柱凝胶法检出DEL型阳性36例(18.75%)。192例患者RhCE表型以ccdee为主,占54.69%(105/192);其次为Ccdee,占31.25%(60/192)。其中,DEL阳性患者以Ccdee为主,占69.44%(25/36),CCdee次之,占25%(9/36);DEL阴性患者以ccdee为主,占66.67%(104/156),Ccdee次之,占22.44%(35/156)。DEL阳性和DEL阴性患者间RhCE表型经χ~2检验分析,χ~2=74.111,P<0.01,二者间差异有统计学意义。结论:本地区RhD阴性人群中存在一定比例的DEL阳型,并且DEL阳性和DEL阴性人群间RhCE表型也存在明显差异,为保障临床输血安全,对RhD阴性的献血者进行DEL型鉴定有重要意义。
Objective: To investigate the distribution of DEL type in RhD-negative patients in this area. Methods: RhD screening was performed on patients with Rh blood type antigen test card, negative RhD negative was confirmed by modified indirect antiglobulin test (IAT), DEL type was detected by absorbance-microcolumn gel method, monoclonal IgM C, C , E, e antisera RhCE phenotype detection. Results: In the 192 specimens of RhD-negative specimens, 36 samples (18.75%) with DEL positive were detected by absorbance-microcolumn gel method. The RhCE phenotype of the 192 patients was mainly ccdee, accounting for 54.69% (105/192), followed by Ccdee (31.25%, 60/192). Among them, the majority of DEL-positive patients were Ccdee, accounting for 69.44% (25/36), followed by CCdee, accounting for 25% (9/36). The majority of DEL-negative patients were ccdee, accounting for 66.67% (104/156) Second, accounting for 22.44% (35/156). RhCE phenotype between DEL positive and DEL negative patients was analyzed byχ ~ 2 test, χ ~ 2 = 74.111, P <0.01, the difference was statistically significant. CONCLUSIONS: There is a certain proportion of DEL positives in RhD negative population in this region, and RhCE phenotypes are also significantly different between DEL positive and DEL negative populations. To ensure the safety of clinical transfusion, it is important to identify DEL type in RhD negative donors significance.