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麦醇溶蛋白在品种间存在明显的差异,其电泳谱带完全受基因控制,几乎不受环境影响,因此被称为品种的指纹,但应用于小麦醇溶蛋白分析的酸性聚丙烯酰胺凝胶采用的是FeSO_4-Vc-H_2O_2催化系统,由该系统催化的凝胶和碱性凝胶相比质量差,胶软,易和玻璃板粘着,卸板很困难。且该系统要求丙烯酰胺和亚甲基丙烯酰胺的纯度较高,限制了其应用范围。国内外对酸性系统改进的不少,但多集中于对缓冲系统的改进和催化系统中各物质量的增减,其结果对凝胶质量改进不大。在本研究中,我们采用新的催化系统FeSO_4-Vc-AP(过硫酸铵),较大提高胶的质量。但因这一催化系统的建立时间尚短,其应用于小麦醇溶蛋白分析的最佳条件还不清楚,本文在这一方面做了一些探索。
Gliadins are significantly different among cultivars and their electrophoresis bands are completely genetically controlled with little environmental impact and hence are referred to as fingerprinting of the cultivars, but the acid polyacrylamide gel used for the analysis of wheat gliadin The FeSO 4 -Vc-H 2 O 2 catalyst system is adopted. Compared with the alkaline gel, the gel catalyzed by the system is inferior in quality, soft in adhesive and easy to adhere to the glass plate, and the unloading of the plate is very difficult. And the system requires higher purity acrylamide and methacrylamide, limiting its scope of application. At home and abroad, many improvements have been made to the acid system, but more attention has been paid to the improvement of the buffer system and the increase or decrease of the amount of each substance in the catalytic system. As a result, the gel quality is not greatly improved. In this study, we use a new catalytic system, FeSO 4 -Vc-AP (ammonium persulfate), to greatly improve the quality of the glue. However, due to the short establishment time of this catalytic system, the optimal conditions for its application to the analysis of prolamin in wheat are not clear. This article has made some explorations in this aspect.