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目的观察去卵巢大鼠血清I型原胶原C-端前肽(PICP)、骨钙素(BGP)和I型胶原交联羧基末端肽(ICTP)的变化,以及骨髓细胞骨保护素(OPG)和核因子κB受体活化因子配体(RANKL)的基因表达变化。方法取3月龄雌性SD大鼠16只,随机分2组:假手术对照组(8只)和假手术组(8只)。采用双侧卵巢切除术复制骨质疏松大鼠模型。术后12周,应用双能X线吸收仪法(DXA)测骨密度,ELISA法测量血清PICP、BGP及ICTP浓度,qRT-PCR法半定量骨髓细胞OPG和RANKL mRNA表达量。结果与假手术组比较,大鼠去卵巢12周后腰椎和股骨骨密度下降显著(P<0.01);血清PICP、BGP和ICTP含量显著升高(P<0.01);骨髓细胞RANKL的mRNA表达量增加(P<0.05)且OPG/RANKL的比率降低(P<0.01),而两者OPG的mRNA表达量无显著性差别(P>0.05)。结论去卵巢12周后的大鼠表现出高骨转换率的特点。OPG/RANKL/RANK系统失衡可能在以破骨细胞功能亢进为特点的骨代谢中起重要作用。
Objective To observe the changes of serum C-terminal propeptide (PICP), osteocalcin (BGP) and type I collagen C-terminal peptide (ICTP) in ovariectomized rats and the effect of osteoprotegerin (OPG) And nuclear factor kappa B receptor activator ligand (RANKL) gene expression changes. Methods Sixteen three-month-old female Sprague-Dawley rats were randomly divided into two groups: sham operation control group (n = 8) and sham operation group (n = 8). Bilateral ovariectomized rat models of osteoporosis were replicated. Twelve weeks after the operation, bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DXA). Serum PICP, BGP and ICTP concentrations were measured by ELISA. MRNA expression of OPG and RANKL in the bone marrow cells was detected by qRT-PCR. Results Compared with the sham group, the BMD of lumbar vertebral and femur decreased significantly after ovariectomy for 12 weeks (P <0.01), and the levels of serum PICP, BGP and ICTP were significantly increased (P <0.01). The mRNA expression of RANKL (P <0.05), and the ratio of OPG / RANKL decreased (P <0.01). There was no significant difference in OPG mRNA expression between the two groups (P> 0.05). Conclusion The rats after ovariectomized for 12 weeks showed the characteristics of high bone turnover rate. The imbalance of the OPG / RANKL / RANK system may play an important role in the metabolism of bone, which is characterized by osteoclastogenesis.