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目的观察β-壳寡糖对糖尿病肾病大鼠肾脏的保护作用,并与α-壳寡糖的肾脏保护作用作比较。方法腹腔注射链脲佐菌素制备糖尿病大鼠模型,6周后测尿蛋白量确定糖尿病肾病模型。给药组分别灌胃给以α-壳寡糖和β-壳寡糖,连续6周。实验第16周末,测24h尿白蛋白、血糖、Scr、Ucr、BUN、SOD、MDA含量及GSH-Px活性。结果β-壳寡糖和α-壳寡糖均能显著降低血糖,且α-壳寡糖的降血糖作用优于β-壳寡糖;二者均能显著降低尿白蛋白、BUN、Scr及MDA,且β-壳寡糖作用显著高于α-壳寡糖;二者均可显著升高SOD和GSH-Px活性,且β-壳寡糖作用显著高于α-壳寡糖。结论β-壳寡糖可降低糖尿病肾病大鼠的血糖,对糖尿病肾病大鼠的肾脏有一定的保护作用。β-壳寡糖的降血糖作用低于α-壳寡糖,肾脏保护作用高于α-壳寡糖。
OBJECTIVE To observe the protective effect of β-oligosaccharide on the kidney of diabetic nephropathy rats, and to compare with the protective effect of α-chito oligosaccharide on the kidney. Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin. After 6 weeks, the amount of urinary protein was measured to determine the diabetic nephropathy model. The administration group were fed with α-chitooligosaccharides and β-chitooligosaccharides continuously for 6 weeks. Urine albumin, blood glucose, Scr, Ucr, BUN, SOD, MDA content and GSH-Px activity were measured at the end of the 16th week. Results Both β-chito oligosaccharide and α-chito oligosaccharide could significantly lower blood glucose, and the hypoglycemic effect of α-chito oligosaccharide was better than that of β-chito oligosaccharide. Both of them could significantly reduce the urinary albumin, BUN, Scr and MDA, and β-chitooligosaccharides were significantly higher than α-chitooligosaccharides; both of them could significantly increase the activity of SOD and GSH-Px, and the effect of β-chitooligosaccharides was significantly higher than that of α-chitooligosaccharides. Conclusion β-oligosaccharide can reduce the blood glucose of diabetic nephropathy rats, which can protect the kidneys of rats with diabetic nephropathy. The hypoglycemic effect of β-chitooligosaccharides is lower than that of α-chitooligosaccharides, and the protective effect of kidney is higher than that of α-chitooligosaccharides.