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常规杂交产生的胡杨为亲本的远缘杂种及其回交的后代。用组织培养法作耐盐鉴定,其耐盐力最高时,NaCl含量不能超过0.6％。根据耐盐性状由多基因决定的理论,筛选耐更高盐分的个体就需要在F_2以后各代的极大量个体中进行。这种筛选事实上是不可能的。因此必须用新的方法解决耐高盐杨树新品种的培育问题。在作物中,应用组织培养,筛选耐盐细胞系,国外已经有一些成功的例子。其中,Nab-ors(1976)通过多步选择法,筛选出了耐高盐的烟草细胞系,并再生了植株;Tyagi(1981)在南洋金花中,用一步选择法,获得了耐盐再生植株。 1982年以来,我们在杨树中开始了这一研究。现在已经在小胡系杨树一些无性系和I-45杨中获得了耐0.6％和1.0％NaCl的细胞
The Populus euphratica produced by conventional hybridization is the distant hybrid of the parent and the backcrossing progeny. Tissue culture for salt tolerance identification, the highest salt tolerance, NaCl content can not exceed 0.6%. Based on the theory of multi-gene resistance to salt tolerance, screening for individuals with higher salinity needs to occur in very large numbers of generations after F_2. This screening is virtually impossible. Therefore, we must use new methods to solve the cultivation of new varieties of high salt tolerant poplar. In crops, the application of tissue culture, screening of salt-tolerant cell lines, there have been some successful foreign examples. Among them, Nab-Ors (1976) screened the tobacco salt tolerant tobacco cell line by multi-step selection method and regenerated the plant; Tyagi (1981) obtained the salt-tolerant regeneration Plant. Since 1982, we started this research in Poplar. Cells that are resistant to 0.6% and 1.0% NaCl have now been obtained in some clones of Populus pruinosa and I-45 poplar