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目的 研究小儿原发性肾病综合征时白细胞介素 1 3(IL 1 3)的变化。方法 对 5 0例肾病综合征患儿和 30例正常健康儿童外周血单个核细胞进行分离、培养 ,并应用双抗体夹心ELISA法检测培养上清中由Th2细胞来源的细胞因子IL 1 3的水平。结果 (1 )原发性肾病综合征患儿急性期外周血单个核细胞培养上清中IL 1 3水平较缓解期和正常对照组显著升高 ,而缓解期降至正常水平。 (2 )肾炎性肾病患儿急性期IL 1 3水平显著高于单纯性肾病患儿。结论 IL 1 3在肾病综合征的发病机制中发挥着重要的作用。外周血单个核细胞培养上清中IL 1 3水平的变化可作为观察病情活动状态的一个免疫学指标。
Objective To study the changes of interleukin-13 (IL-13) in children with primary nephrotic syndrome. Methods Peripheral blood mononuclear cells from 50 children with nephrotic syndrome and 30 normal healthy children were isolated and cultured. The level of cytokine IL-13 derived from Th2 cells in culture supernatant was detected by double antibody sandwich ELISA . Results (1) The level of IL-13 in the peripheral blood mononuclear cell culture supernatant of patients with primary nephrotic syndrome was significantly higher than that of the normal control group and normal control group, while the remission period decreased to normal level. (2) The level of IL-13 in children with nephritis is significantly higher than that in children with simple nephropathy. Conclusion IL 1 3 plays an important role in the pathogenesis of nephrotic syndrome. The change of IL-13 level in peripheral blood mononuclear cell culture supernatants can be used as an immunological indicator to observe the status of disease activity.