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目的 探讨电离辐射对大鼠脑内Fos蛋白表达的早期影响。 方法 应用大鼠半脑 2 0Gy照射模型 ,免疫组织化学方法 ,观察Fos蛋白在脑内的表达及分布情况。 结果 受照射后 2 4h、1周大鼠脑内各部位Fos蛋白表达均明显减少 ,随着时间的延长 ,其Fos免疫反应性细胞数量逐渐增加 ,照射后 4周 ,延髓、脑桥内Fos免疫阳性细胞数量恢复并超过正常对照组水平 ,但中脑、间脑及端脑内未恢复到正常对照组水平。 结论 结果提示 :中等剂量辐射后早期 ,脑内许多核团的神经细胞功能活动可能受到抑制 ,并且越高级的中枢受到的抑制影响越明显
Objective To investigate the early effects of ionizing radiation on Fos protein expression in rat brain. Methods The rat model of 20 Gy irradiation and immunohistochemistry were used to observe the expression and distribution of Fos protein in the brain. Results The expression of Fos protein in various parts of the brain decreased significantly at 24 h and 1 week after irradiation, and the number of Fos immunoreactive cells increased gradually with time. At 4 weeks after irradiation, Fos immunoreactivity in medulla oblongata and pons was positive The number of cells recovered and exceeded the level of normal control group, but the level of normal control group was not recovered in midbrain, midbrain and telencephalon. Conclusions The results suggest that early in the middle dose of radiation, the functional activity of many nerve cells in the brain may be inhibited, and the effect of the inhibition of the more advanced central is more obvious