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目的探讨解聚素金属蛋白酶(a disintegrin and metalloproteinase domain,ADAM)家族中ADAM8、15、19在类风湿关节炎(RA)中的表达研究。方法使用实时荧光定量PCR(real-time PCR)技术检测RA患者、骨关节炎(osteoarthritis,OA)患者和痛风性关节炎(gouty arthritis,GA)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中ADAM8、15和19的表达,并且和疾病的炎症活动指标及标志性抗体进行相关分析,疾病炎症活动相关指标包括红细胞沉降率(erythrocytesedimentationrate,ESR)、C反应蛋白(C reactive protein,CRP),此外还包括RA的类风湿因子(rheumatoid factor,RF)和抗环瓜氨酸肽(anti-citrullinatedprotein antibody,anti-CCP)抗体。结果与对照者相比,ADAM8在RA、OA及GA组均增高,而正常对照者表达水平较低,差异有统计学意义(P=0.0001);ADAM8和疾病的活动性指标CRP具有相关性(r=0.330,P=0.002);ADAM15在RA、OA及GA中表达增高,但在RA中的表达升高尤其明显,差异有统计学意义(P=0.0035),ADAM15和RA的RF具有相关性(r=0.319,P=0.016);ADAM19只在RA中表达增高,差异有统计学意义(P=0.0086),ADAM19和RA的RF具有相关性(r=0.314,P=0.018)。结论 ADAM8参与了RA、OA和GA的发病过程,并与上述3种关节炎的活动性指标具有相关性。ADAM15及ADAM19主要参与了RA的发病,且ADAM15、ADAM19与RA的RF具有相关性。
Objective To investigate the expression of ADAM8, 15 and 19 in rheumatoid arthritis (RA) in a disintegrin and metalloproteinase domain (ADAM) family. Methods Real-time PCR was used to detect the expression of peripheral blood mononuclear cells (PBMCs) in patients with RA, osteoarthritis (OA) and gouty arthritis (GA) ), ADAM8, 15 and 19 were detected and correlated with the inflammatory activity index and the marker antibody of the disease. The index of disease inflammatory activity included erythrocyte sedimentation rate (ESR), C reactive protein (CRP) , In addition to RA’s rheumatoid factor (RF) and anti-citrullinated protein anti-CCP antibodies. Results Compared with the controls, ADAM8 increased in RA, OA and GA groups, while the expression in normal controls was lower (P = 0.0001); ADAM8 correlated with activity of CRP r = 0.330, P = 0.002). The expression of ADAM15 was increased in RA, OA and GA, but the expression was significantly increased in RA (P = 0.0035). There was a correlation between ADAM15 and RF in RA (r = 0.319, P = 0.016). The expression of ADAM19 was only increased in RA with statistical significance (P = 0.0086). The AD of ADAM19 was correlated with that of RA (r = 0.314, P = 0.018). Conclusion ADAM8 is involved in the pathogenesis of RA, OA and GA, and is related to the above three indexes of arthritis activity. ADAM15 and ADAM19 are mainly involved in the pathogenesis of RA, and ADAM15, ADAM19 and RF of RF have relevance.