论文部分内容阅读
目的探讨ＨＢＶ侵犯外周血单个核细胞（ＰＢＭＣ）后对其线粒体功能的影响．方法ＨＢｓＡｇ阳性６个月以上，无肝炎症状及体征，肝功正常患者５８例．多聚酶链反应检测ＰＢＭＣ中ＨＢＶＤＮＡ，噻唑兰（ＭＴＴ）比色法测线粒体功能．ＨＢｓＡｇ、ＨＢｅＡｇ检测用固相放免法检测．结果慢性ＨＢＶ感染者５８例，ＰＢＭＣ中检出ＨＢＶＤＮＡ３１例（５３５％），ＰＢＭＣ中ＨＢＶＤＮＡ阳性组ＭＴＴ比色法查线粒体功能的Ａ（ＯＤ５００ｎｍ）值明显低于ＨＢＶＤＮＡ阴性组（００５±００３ｖｓ０２９±００７，Ｐ＜００１）．结论慢性ＨＢＶ感染时，ＨＢＶ常侵犯ＰＢＭＣ，并导致ＰＢＭＣ线粒体功能降低、能量代谢异常．这可为ＨＢＶ慢性感染免疫功能异常的原因之一．
Objective To investigate the effect of HBV on the mitochondrial function of peripheral blood mononuclear cells (PBMCs). Methods HBsAg positive for more than 6 months, no symptoms and signs of hepatitis, normal liver function in 58 patients. Polymerase chain reaction detection of PBMC in HBVDNA, thiazole blue (MTT) colorimetric mitochondrial function. HBsAg, HBeAg detection using solid phase radioimmunoassay. Results There were 58 cases with chronic HBV infection and 31 cases (535%) with HBVDNA in PBMC. The mitochondrial function A (OD500nm) value of HBVDNA positive group in PBMC was significantly lower than that in HBVDNA negative group (005 ± 003vs029 ± 007, P <001). Conclusion HBV infection often invades PBMC during chronic HBV infection and leads to mitochondrial dysfunction and energy metabolism in PBMC. This may be one of the reasons for the abnormal immune function of chronic HBV infection.