本文采用七种活性染料为亲和配体,在双水相系统中分离人血清白蛋白、α球蛋白和β-球蛋白。实验结果表明这七种染料配体对白蛋白和β-球蛋白具有一定的亲和作用,对α-球蛋白的亲和力不显著。其中,配体活性金黄K-MG对所研究的蛋白质具有良好的亲和作用和选择性,是一种具有较高应用价值的染料配体。 本文还探讨了pH值、盐及温度等因素对三种蛋白质分配效果的影响。 In this paper, seven kinds of reactive dyes were used as affinity ligands to separate human serum albumin, alpha globulin and beta-globulin in a two-aqueous system. The experimental results show that the seven dye ligands have some affinity to albumin and β-globulin, and have no significant affinity to α-globulin. Among them, the ligand-active golden K-MG has good affinity and selectivity for the studied protein, and is a kind of dye ligand with high application value. This article also discussed the pH, salt and temperature and other factors on the distribution of three kinds of protein effects.