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目的研究人参皂苷水解产物DS-1227(Dammarane Sapogenins-1227)对东莨菪碱致小鼠学习记忆障碍的改善作用,为老年痴呆等疾病的药物研究提供科学数据。方法将60只ICR小鼠随机分为空白对照组、模型组、阳性对照组、DS-1227低、中、高剂量组共6组,每组10只。连续灌胃给药2周后,检测自主活动,第15天开始除空白对照组腹腔注射生理盐水,其他各组均腹腔注射东莨菪碱(0.75 mg/kg),阳性对照组在注射东莨菪碱5min后另腹腔注射毒扁豆碱(0.3 mg/kg),给药完成20 min后进行物体认知实验和Morris水迷宫实验检测动物的学习记忆能力。结果 DS-1227对小鼠的自主活动无显著性影响。腹腔注射东莨菪碱后,小鼠的相对辨别指数降低,水迷宫定位航行实验中d1、d2、d3、d4和d5潜伏期均明显延长。DS-1227低、中、高剂量和给予M受体激动剂毒扁豆碱均能不同程度增加东莨菪碱致学习记忆障碍模型小鼠的相对辨别指数,缩短水迷宫定位航行潜伏期。结论DS-1227有改善东莨菪碱致小鼠学习记忆障碍的作用。
Objective To study the effect of DS-1227 (Dammarane Sapogenins-1227) on learning and memory impairment caused by scopolamine in mice and provide scientific data for drug research on diseases such as Alzheimer’s disease. Methods Sixty ICR mice were randomly divided into blank control group, model group, positive control group, DS-1227 low, medium and high dose groups of 6 groups, 10 in each group. After 2 weeks of continuous intragastric administration, autonomic activity was measured. On the 15th day, all rats in the control group were given intraperitoneal injection of scopolamine (0.75 mg / kg) except for the blank control group. The positive control group received intraperitoneal injection of scopolamine After injection of physostigmine (0.3 mg / kg), cognitive learning and Morris water maze test were used to detect the learning and memory abilities of the animals 20 minutes after the completion of the administration. Results DS-1227 had no significant effect on autonomic activity in mice. After sc injection of scopolamine intraperitoneally, the relative discrimination index of mice decreased, and the latencies of d1, d2, d3, d4 and d5 in water maze positioning navigation experiment were significantly prolonged. DS-1227 low, medium and high doses and administration of physostigmine physostigmine could all increase the relative discrimination index of scopolamine-induced learning and memory impairment mice to some extent and shorten the water maze positioning navigation latency. Conclusion DS-1227 can improve scopolamine-induced learning and memory impairment in mice.