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目的:改进中国药典一部收载的三种百部的鉴别方法。方法:利用性状鉴别、显微鉴别与HPLC色谱鉴别方法对直立百部、蔓生百部、对叶百部进行鉴别。结果:粉末显微鉴别中直立百部、蔓生百部的根被细胞壁木栓化及木化,具致密的细条纹,对叶百部根被细胞壁无细条纹;HPLC色谱中直立百部、蔓生百部含有原百部碱,对叶百部中不含原百部碱。结论:该研究结果为中国药典进一步修订,建立不同基源百部的专属性鉴别提供了实验依据。
Objective: To improve the identification of a Chinese Pharmacopoeia containing three kinds of hundred parts. Methods: The characteristics of identification, microscopic identification and HPLC chromatographic identification method of erect 100, 100 parts of vine, to identify the leaves. Results: In the microscopic examination of powder, there were 100 upright stems, roots of 100 stems were corked and woody with dense fine stripes, Hundreds contain the original alkaloid, the leaves do not contain the original 100 parts of alkali. CONCLUSION: The results of this study provide experimental basis for the further revision of Chinese Pharmacopoeia and establishment of the identification of the specialties of different genotypes.