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干旱胁迫下的脯氨酸积累在许多植物中都存在,人们普遍认为脯氨酸含量的提高促进了植物对干旱胁迫的抵抗能力。拟南芥中研究发现,△1-吡咯啉-5-羧酸合成酶基因P5CS1是一种限速酶用来催化脯氨酸生物合成,对脯氨酸非常重要。本研究通过生物信息学及荧光定量PCR对大豆中的P5CS1同源基因Glyma01g24530进行了初步的功能分析和表达模式验证。结果发现:Glyma01g24530具有保守的N端乙酰谷氨酸合成酶激酶结构域和N端乙酰谷氨酸合成酶激酶结构域,系统进化树显示与各植物中P5CS家族成员相似性很高,启动子顺式作用元件序列分析表明该基因存在逆境胁迫、光反应等元件。表达模式分析显示Glyma01g24530可以被干旱、盐诱导表达,并在多数组织中表达。
Proline accumulation under drought stress is present in many plants, and it is generally accepted that an increase in proline content promotes plant resistance to drought stress. In Arabidopsis, it has been found that the Δ1-pyrroline-5-carboxylic acid synthase gene P5CS1 is a rate-limiting enzyme that catalyzes proline biosynthesis and is very important for proline. In this study, bioinformatics and real-time PCR were used to characterize the P5CS1 homolog Glyma01g24530 in soybean. The results showed that Glyma01g24530 has a conserved N-terminal acetylglutamate synthase kinase domain and an N-terminal acetylglutamate synthase kinase domain. Phylogenetic tree showed high homology with P5CS family members in each plant. Sequence analysis of the functional elements showed that the gene has stress, light response and other components. Expression pattern analysis showed that Glyma01g24530 can be induced by drought, salt and expressed in most tissues.