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目的 观察剂量及极低频电磁场对睫状神经营养因子 (CNTF)、白细胞介素 1α(IL 1α)诱导新生大鼠中脑神经干细胞 (NSC)分化的影响。 方法 NSC接受不同剂量CNTF、IL 1α和极低频电磁场处理 5d ,利用神经元特异性标记物微管相关蛋白 2ab(MAP2ab) ,通过免疫荧光化学染色检测神经干细胞神经元向的分化百分比。结果与对照组相比 ,小剂量CNTF和IL 1α组NSC神经元向分化百分比增加 ;大剂量CNTF组该比例明显降低 (P <0 0 1)。施加电磁场后 ,小剂量CNTF的促进效应增强 ,尤其是电磁场可使大剂量CNTF的神经元向分化抑制作用转变为促进作用。电磁场抑制了小剂量IL 1的促进作用 ,不影响大剂量IL 1的诱导结果。 结论 细胞因子对NSC的诱导分化结果与剂量有关。极低频电磁场与细胞因子的共同效应不是简单协同或拮抗 ,说明生物磁场在神经系统发育中的作用不可忽视 ,电磁场可能成为调控NSCs分化的新手段。
Objective To observe the effects of dose and ultra-low frequency electromagnetic fields on the differentiation of neural stem cells (NSC) in cortex of neonatal rats induced by ciliary neurotrophic factor (CNTF) and interleukin-1α (IL-1α). Methods NSCs were treated with different doses of CNTF, IL-1α and ultra-low frequency electromagnetic fields for 5 days. Neuronal differentiation percentage of neural stem cells was detected by immunofluorescence staining with neuron-specific marker 2ab (MAP2ab). Results Compared with the control group, the percentage of NSC neurons in the low-dose CNTF and IL-1α groups increased. The proportion of high-dose CNTF group was significantly decreased (P <0.01). After the application of the electromagnetic field, the promotion effect of the small dose of CNTF is enhanced. Especially, the electromagnetic field can convert the neurons of the large dose of CNTF to the differentiation inhibition effect into the promotion effect. Electromagnetic fields inhibited the promotion of low-dose IL-1 without affecting the induction of high-dose IL-1. Conclusion The results of cytokine induced differentiation of NSC are related to dosage. The common effect of ELF and cytokines is not simple synergism or antagonism, indicating that the role of biological magnetic fields in the development of the nervous system can not be neglected. Electromagnetic fields may become new means to regulate the differentiation of NSCs.