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目的 :克隆、表达HPV16湖北株E7基因并探讨E7蛋白的免疫学性质。方法 :应用基因克隆技术克隆、表达HPV16E7基因 ,纯化E7蛋白并免疫动物 ,用ELISA检测动物血清中的特异性E7抗体 ,进而评估E7蛋白的抗原性。结果 :融合型的E7蛋白产量可达细胞总蛋白量的 30 % ;ELISA检测表明动物体内产生了特异性E7抗体。结论 :HPV16湖北株E7蛋白可通过基因克隆方法制备 ,并保留了标准E7蛋白的抗原性
OBJECTIVE: To clone and express E6 gene of HPV16 Hubei strain and investigate the immunological properties of E7 protein. Methods: HPV16E7 gene was cloned and expressed by gene cloning technique. E7 protein was purified and immunized. ELISA was used to detect the specific E7 antibody in animal serum to evaluate the antigenicity of E7 protein. Results: The yield of fusion E7 protein reached 30% of the total cellular protein. ELISA assay showed that specific E7 antibody was produced in animals. Conclusion: HPV16 Hubei strain E7 protein can be prepared by gene cloning method and retain the antigenicity of standard E7 protein